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Pacific Northwest National Laboratory researchers use the newest flow cytometer, FACS-ARIA, to isolate and characterize antibodies. The machine is unparalleled in speed and utility, requires less operator time, and provides a higher fluorescent and sensitivity resolution.

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Document Title: Flow Cytometry
Category: Biomolecular Systems and Science
Media Type: Photos
Date of Image/Photo: September 2003
Background: Flow cytometry allows high throughput single cell investigation. Routinely used in the Microbial Cell Dynamics Laboratory at the Pacific Northwest National Laboratory (PNNL), it rapidly interrogates bacteria, yeast and mammalian cell populations on a single cell basis. PNNL is one of a few laboratories in the world that uses flow cytometry to isolate and characterize antibodies. Fluorescent activated cell sorting (FACS) has become an indispensable tool in biology. Recent technical advances allow fine resolution between cell types by measuring many optical parameters in parallel. FACS provides the ability to separate a heterogeneous suspension of cells into a purified fraction on the basis of fluorescence and light scattering properties. To facilitate this cell research, PNNL has purchased the latest in flow cytometers, the FACS-ARIA from BD Biosciences. This state-of-the-art equipment provides a single cell sorting capability at a rate of up to 70,000 events onto a variety of formats. PNNL will use the FACS-ARIA to calibrate, analyze, and isolate cells of interest. Creating affinity reagents to important biomolecules is one of the most critical and challenging tasks facing biologists. PNNL was the first to create an immense library of non-immune antibodies to be displayed on the surface of the yeast, Saccharonmyces cerevisiae. A unique combination of technologies allows antibodies present in this library to bind to specific antigens that are readily isolated after adding a fluorescently-labeled antigen and using the new FACS-ARIA cell sorter.
URL of this page: http://picturethis.pnl.gov/picturet.nsf/by+id/AMER-5S6VPG

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